Matches in Nanopublications for { ?s ?p "mitochondria-enriched fractions prepared from these cells were lysed and subjected to SDS-PAGE. Even if mtNOS is constitutively expressed, the levels seem to be low, since no iNOS immunoreactivity was detected in the absence of LPS and under experimental conditions used (Figure ?(Figure6C).6C). However, in the mitochondrial fraction prepared from LPS-exposed ECs, mtNOS protein expression is easily detected.....mtNOS activity was assessed by exposing freshly prepared, unlysed mitochondrial fractions from ECs to oxyHb, as described under Methods. The rate of NO production was measured by the change in optical density of the reaction mixture as generated NO quickly oxidized oxyHb to metHb. As shown in Figure ?Figure6D,6D, mitochondria from LPS-treated ECs exhibited a two-fold greater rate of NO formation than untreated controls" ?g. }
Showing items 1 to 1 of
1
with 100 items per page.
- _2 value "mitochondria-enriched fractions prepared from these cells were lysed and subjected to SDS-PAGE. Even if mtNOS is constitutively expressed, the levels seem to be low, since no iNOS immunoreactivity was detected in the absence of LPS and under experimental conditions used (Figure ?(Figure6C).6C). However, in the mitochondrial fraction prepared from LPS-exposed ECs, mtNOS protein expression is easily detected.....mtNOS activity was assessed by exposing freshly prepared, unlysed mitochondrial fractions from ECs to oxyHb, as described under Methods. The rate of NO production was measured by the change in optical density of the reaction mixture as generated NO quickly oxidized oxyHb to metHb. As shown in Figure ?Figure6D,6D, mitochondria from LPS-treated ECs exhibited a two-fold greater rate of NO formation than untreated controls" provenance.