Matches in Nanopublications for { ?s <http://www.w3.org/ns/prov#value> ?o ?g. }
- _5 value "Increased protein tyrosine phosphatase activity as well as mRNA levels of PTPbeta2 and PTPepsilon could be observed by wildtype p53 overexpression." provenance.
- _5 value "We show here that ILK overexpression elevates cyclin D1 protein levels and directly induces the cyclin D1 gene in mammary epithelial cells. I" provenance.
- _5 value "Wnt-1 overexpression in mammary epithelial cells induced cyclin D1 mRNA and targeted overexpression of Wnt-1 in the mammary gland of transgenic mice increased both ILK activity and cyclin D1 levels." provenance.
- _6 value "ERK1, and ERK2 phosphorylates and activates to SREBP1" provenance.
- _6 value "ERK1, and ERK2 phosphorylates and activates to SREBP1" provenance.
- _5 value "Mutation of serine 117 to alanine abolished Erk2-mediated phosphorylation in vitro and the MAP kinase-related transcriptional activation of SREBP-1a by insulin and platelet-derived growth factor in vivo." provenance.
- _3 value "antiproliferative effects of IFN-beta than of IFN-alpha" provenance.
- _4 value "Modified assertion" provenance.
- _3 value "Hypertonicity provoked Fyn-dependent tyrosine phosphorylation in beta-catenin, alpha-catenin, and p120(Cas) and caused the dissociation of beta-catenin from the contacts." provenance.
- _3 value "Hypertonicity provoked Fyn-dependent tyrosine phosphorylation in beta-catenin, alpha-catenin, and p120(Cas) and caused the dissociation of beta-catenin from the contacts." provenance.
- _10 value "Duplin bound directly to the Armadillo repeats of beta-catenin, thereby inhibiting the binding of Tcf to beta-catenin." provenance.
- _6 value "In response to Wnt, beta-catenin is stabilized and translocated into the nucleus where it stimulates gene expression through Tcf/Lef." provenance.
- _4 value "Insulin receptor tyrosine kinase activity was significantly decreased in skeletal muscle from obese insulin resistant patients compared with lean insulin sensitive patients. Tyrosine kinase activity of insulin receptor was increased by 48% in obese insulin resistant skeletal muscle and was not significantly changed in lean subjects by alkaline phosphatase treatment. Moreover increased membrane associated PKC activity and PKC-beta translocation to membrane was induced by insulin treatment in muscle fibers of obese subjects. These results suggested that increased serine/threonine phosphorylation of insulin receptor mediated by PKC isoforms (beta, theta, delta) is responsible for reduced insulin receptor tyrosine kinase activity in obese subjects." provenance.
- _6 value "Modified assertion" provenance.
- _6 value "Modified assertion" provenance.
- _4 value "inhibition of CLN3 protein synthesis by antisense approach acidifies lysosomal compartments. These changes in lysosomal pH are sufficient to exert a significant biological effect and modify intracellular processing of amyloid-beta protein precursor and cathepsin D, model proteins whose metabolism is influenced by the pH of acidic organelles." provenance.
- _4 value "Also, stimulation with TGF-b1 (1 ng/ml) caused a time-dependent increase in IL-6 protein accumulation in the culture supernatants (Fig. 2C)." provenance.
- _3 value "Angiotensin II induces apoptosis of human endothelial cells: protective effect of nitric oxide" provenance.
- _5 value "Significantly, phosphorylation of hPOMp100/PSF by protein kinase C inhibits its binding to RNA but stimulates its binding to DNA and D-loop formation and may represent a regulatory mechanism to direct this multifunctional protein to DNA metabolic pathways." provenance.
- _3 value "Muscle-G4KO mice showed insulin resistance" provenance.
- _8 value "Furthermore, Iba1 colocalized with a small GTPase Rac in the membrane ruffles and the phagocytic cups. The Iba1 mutants also suppressed membrane ruffling induced by dominant active Rac1V12" provenance.
- _3 value "JunB, c-Jun, c-Fos, and Fra2 were rapidly but transiently induced by FSH in immature granulosa cells." provenance.
- _3 value "JunB, c-Jun, c-Fos, and Fra2 were rapidly but transiently induced by FSH in immature granulosa cells." provenance.
- _3 value "TNF-alpha-induced ROS generation was blocked by inhibition of cPLA(2) or 5-LO, but not cyclooxygenase, suggesting that TNF-alpha-induced ROS generation is dependent on synthesis of AA and its subsequent metabolism to leukotrienes." provenance.
- _5 value "Modified assertion" provenance.
- _6 value "expression of CDK4/cyclin D1, which stimulated RB phosphorylation" provenance.
- _6 value "Jubilant: Following the treatment with IL-18 plus IL-12, IFNgamma and TNFalpha levels rose rapidly, GM-CSF modestly elevated, Il-1beta, IL-6 and IL-10 were markedly elevated in SCID mice. IL-18 alone did not stimulate significant endogenous production of IFNgamma, TNFalpha and GM-CSF, whereas modest serum level of IFNgamma were observed in mice receiving injections of IL-12 alone." provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "In addition to reduced expression of the genes encoding insulin, glucose transporter-2, L-pyruvate kinase, aldolase B and 3-hydroxy-3-methylglutaryl coenzyme A reductase, induction of HNF1 alpha-P291fsinsC also significantly inhibits expression of mitochondrial 2-oxoglutarate dehydrogenase (OGDH) E1 subunit mRNA and protein. OGDH enzyme activity and [(14)C]pyruvate oxidation were also reduced. In contrast, the mRNA and protein levels of mitochondrial uncoupling protein-2 were dramatically increased by HNF1 alpha-P291fsinsC induction." provenance.
- _5 value "In addition to reduced expression of the genes encoding insulin, glucose transporter-2, L-pyruvate kinase, aldolase B and 3-hydroxy-3-methylglutaryl coenzyme A reductase, induction of HNF1 alpha-P291fsinsC also significantly inhibits expression of mitochondrial 2-oxoglutarate dehydrogenase (OGDH) E1 subunit mRNA and protein. OGDH enzyme activity and [(14)C]pyruvate oxidation were also reduced. In contrast, the mRNA and protein levels of mitochondrial uncoupling protein-2 were dramatically increased by HNF1 alpha-P291fsinsC induction." provenance.
- _4 value "FGF-2 stimulated DNA synthesis and induced a sustained phosphorylation of p42/44(MAPK) and of its downstream target, p90(RSK)." provenance.
- _4 value "FGF-2 stimulated DNA synthesis and induced a sustained phosphorylation of p42/44(MAPK) and of its downstream target, p90(RSK)." provenance.
- _5 value "Axam inhibited the complex formation of Dvl with Axin and the activity of Dvl to suppress GSK-3beta-dependent phosphorylation of Axin." provenance.
- _6 value "Modified assertion" provenance.
- _5 value "In response to PDGF, Fyn associated with PKCdelta via tyrosine 187. Finally, overexpression of dominant negative Fyn abrogated the decrease in GS expression and reduced the tyrosine phosphorylation of PKCdelta induced by PDGF. We conclude that the tyrosine phosphorylation of PKCdelta and its association with tyrosine kinases may be an important point of divergence in PKC signaling." provenance.
- _5 value "Modified assertion" provenance.
- _4 value "Further, IL-13 was most potent at reducing RA ST explant ENA-78 levels compared with other inflammatory mediators that we examined (Fig. 3 B )." provenance.
- _5 value "Semiquantitative RT-PCR experiments suggest that the stimulation of cAMPspecific PDE4 is the consequence of an increased mRNA steady state and protein synthesis, at least in the case of PDE isoforms encoded by the 4C and 4D genes" provenance.
- _6 value "Semiquantitative RT-PCR experiments suggest that the stimulation of cAMPspecific PDE4 is the consequence of an increased mRNA steady state and protein synthesis, at least in the case of PDE isoforms encoded by the 4C and 4D genes" provenance.
- _6 value "Semiquantitative RT-PCR experiments suggest that the stimulation of cAMPspecific PDE4 is the consequence of an increased mRNA steady state and protein synthesis, at least in the case of PDE isoforms encoded by the 4C and 4D genes" provenance.
- _6 value "beta- and alpha 2-adrenergic receptors (ARs) couple positively and negatively, respectively, to adenylyl cyclase" provenance.
- _4 value "increased alpha 2/beta-AR balance in adipocytes promotes obesity by stimulating adipocyte hyperplasia" provenance.
- _4 value "SOCS-1 inhibits the activity of JAK1 and hence inhibits the JAK-STAT pathway of IL-4 signaling." provenance.
- _5 value "Taken together, our data indicate that PAR-1 is both necessary and sufficient for mediating the stimulatory effects of thrombin on CTGF/FISP12 mRNA levels. They further show that these stimulatory effects are not restricted to human fibroblasts and confirm the critical role of PAR-1 in mediating the effects of thrombin on mesenchymal cell function." provenance.
- _5 value "Metal response element-binding transcription factor (MTF)-1 mediates metal regulation of the metallothionein I gene. In vitro DNA-binding assays demonstrated that mouse MTF-1 can bind avidly to the two metal-response element sequences found in the ZnT1 promoter. Using mouse embryo fibroblasts with homozygous deletions of the MTF-1 gene, it was shown that this transcription factor is essential for basal as well as metal (zinc and cadmium) regulation of the ZnT1 gene in these cells." provenance.
- _4 value ". Insulin also increases the uptake of fatty acids derived from circulating lipoproteins by stimulating lipoprotein lipase activity in adipose tissue." provenance.
- _2 value "Elevated FFAs impair the ability of insulin to suppress hepatic glucose output and to stimulate glucose uptake into skeletal muscle, as well as to inhibit insulin secretion from pancreatic b cells." provenance.
- _4 value "In all forms of obesity and diabetes, a major factor contributing to the impaired insulin-stimulated glucose transport in adipocytes is the downregulation of GLUT4." provenance.
- _2 value "Insulin promotes adipocyte triglyceride stores by a number of mechanisms, including fostering the differentiation of preadipocytes to adipocytes and, in mature adipocytes, stimulating glucose transport and triglyceride synthesis (lipogenesis), as well as inhibiting lipolysis" provenance.
- _4 value "PPARg is activated by TZDs,which bind to the ligand-binding domain and increase transactivation of target genes. The greatest expression of PPARg is in adipocytes" provenance.
- _4 value "The transcription factor ADD-1/SREBP-1c (adipocyte determination and differentiation factor-1/sterol regulatory element-binding protein-1c) may play a critical role in the actions of insulin to regulate adipocyte gene expression (10-12), by inducing genes involved in lipogenesis and repressing those involved in fatty acid oxidation." provenance.
- _4 value "The transcription factor ADD-1/SREBP-1c (adipocyte determination and differentiation factor-1/sterol regulatory element-binding protein-1c) may play a critical role in the actions of insulin to regulate adipocyte gene expression (10-12), by inducing genes involved in lipogenesis and repressing those involved in fatty acid oxidation." provenance.
- _4 value "rodents or humans are treated with the b3 adrenergic agonist CL316,243 (25, 26). Since b3 adrenergic receptors are expressed almost exclusively in fat, effects of these agents would be expected to be initiated by alterations in fat. Treatment with CL316,243 results in enhanced sensitivity of both whole body glucose uptake and suppression of hepatic glucose production (25). Thus, increasing glucose uptake selectively in fat with b3 adrenergic receptor agonists may improve whole body glucose uptake, with the effects in fat indirectly resulting in increased insulin sensitivity in liver." provenance.
- _5 value "we demonstrated that both HBP1 and RB were involved in the activation of H1(0) gene expression." provenance.
- _6 value "PKB phosphorylation and activity were reduced to the same extent as they were with PTEN expression. Finally we found that SHIP-2, like PTEN, caused a potent cell cycle arrest in G(1) in glioblastoma cells, which is associated with an increase in the stability of expression of the cell cycle inhibitor p27(KIP1) In these studies PtdIns(3,4,5)P3 and PtdIns(3,4)P2 were equally effective at allowing phosphorylation by PDK-1" provenance.
- _5 value "Reexpression of PTEN or treatment with the PI3K inhibitor LY294002 abolished the levels of both PtdIns(3, 4)P2 and PtdIns(3,4,5)P3, reduced phosphorylation of PKB on Thr308 and Ser473, and inhibited PKB activity." provenance.
- _4 value "The multinuclear cells formed after IL-7 stimulation was found to express pp60 (c-src) an authentic marker for human osteoclasts." provenance.
- _4 value "The multinuclear cells formed after IL-7 stimulation was found to increase the expression of calcitonin, an authentic marker for human osteoclasts by 200 fold." provenance.
- _10 value "We report here that c-Cbl, which is a Gab1-like docking protein, also becomes tyrosine-phosphorylated in response to Met activation and serves as a docking molecule for various SH2-containing molecules, including Crk. We further show that Cbl is similarly capable of enhancing Met-induced JNK activation, and several lines of experimentation suggests that it does so by interacting with Crk. " provenance.
- _7 value "# Ariadne: Whereas collagen and fibronectin are the ligands for several eta 1 integrins, other bone matrix proteins, such as osteopontin, bone sialoprotein, and vitronectin can interact with alpha v eta 5 ( 33-36 ). [Binding]" provenance.
- _5 value "The autophosphorylation rate was dependent on Abl protein concentration consistent with an intermolecular reaction." provenance.
- _7 value "Furthermore, growth factor stimulation of these RTKs promotes increased cell proliferation and migration when cells are attached to the alpha(v)beta(3) ligand, vitronectin." provenance.
- _6 value "In vitro the GAP domain activates RhoA, Rac1, and Cdc42 GTPases." provenance.
- _6 value "In vitro the GAP domain activates RhoA, Rac1, and Cdc42 GTPases." provenance.
- _3 value "Chronic elevated glucose reduces the expression levels of the PPAR target genes, uncoupling protein 2 and acyl-CoA oxidase" provenance.
- _3 value "Chronic elevated glucose reduces the expression levels of the PPAR target genes, uncoupling protein 2 and acyl-CoA oxidase" provenance.
- _4 value "Glucose also causes quantitatively similar reductions in PPARalpha protein and DNA binding activity of this transcription factor." provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "Modified assertion" provenance.
- _4 value "Here, we demonstrate that in astrocyte cultures IGF-I regulates NFkappaB, a transcription factor known to play a key role in the inflammatory reaction. IGF-I induces a site-specific dephosphorylation of IkappaBalpha (phospho-Ser(32)) in astrocytes." provenance.
- _5 value "Phosphorylation of Akt is regulated positively by Btk and Syk and negatively by Lyn. Akt in turn can regulate positively the transcriptional activity of interleukin (IL)-2 and tumor necrosis factor (TNF)-alpha promoters." provenance.
- _6 value "Phosphorylation of Akt is regulated positively by Btk and Syk and negatively by Lyn. Akt in turn can regulate positively the transcriptional activity of interleukin (IL)-2 and tumor necrosis factor (TNF)-alpha promoters." provenance.
- _4 value "Aldehyde oxidase (AOX) is a member of the molybdenum iron-sulfur flavoproteins and is of interest for its role in clinical drug metabolism and as a source of reactive oxygen species (ROS) potentially involved in human pathology." provenance.
- _4 value "Tissue levels of IL-1b, TNF-a, and MIP-2 were significantly elevated in the lungs after administration of doses of HMG-1 as low as 1 mg/mouse (Fig. 2)." provenance.
- _4 value "TGF-Beta1 at a concentration of 2 ng/ml induced a 10-fold increase in the level of CXCR4 expressed on synovial CD4 T cells after 48 hours of incubation." provenance.
- _6 value "In addition, introduction of B-Raf enhances and sustains integrin-mediated activation of ERK in wild-type primary fibroblasts" provenance.
- _3 value "Modified assertion" provenance.
- _4 value "Modified assertion" provenance.
- _7 value "Accordingly, pull down assays with human HepG2 cell lysates showed that IR-specific phosphorylation of wild-type hGab-1 strongly enhanced PI-3 kinase binding. " provenance.
- _5 value "Our results demonstrate that hGab-1 was phosphorylated by IR at eight tyrosine residues (Y242, Y285, Y373, Y447, Y472, Y619, Y657, and Y689). Seventy-five percent of the identified radioactivity was incorporated into tyrosine residues Y447, Y472, and Y619 exhibiting features (NYVPM motif) of potential binding sites for the regulatory subunit (p85) of phosphatidylinositol (PI)-3 kinase." provenance.
- _5 value "Our results demonstrate that hGab-1 was phosphorylated by IR at eight tyrosine residues (Y242, Y285, Y373, Y447, Y472, Y619, Y657, and Y689). Seventy-five percent of the identified radioactivity was incorporated into tyrosine residues Y447, Y472, and Y619 exhibiting features (NYVPM motif) of potential binding sites for the regulatory subunit (p85) of phosphatidylinositol (PI)-3 kinase." provenance.
- _5 value "Our results demonstrate that hGab-1 was phosphorylated by IR at eight tyrosine residues (Y242, Y285, Y373, Y447, Y472, Y619, Y657, and Y689). Seventy-five percent of the identified radioactivity was incorporated into tyrosine residues Y447, Y472, and Y619 exhibiting features (NYVPM motif) of potential binding sites for the regulatory subunit (p85) of phosphatidylinositol (PI)-3 kinase." provenance.
- _4 value "Cells treated with Src family kinase inhibitors PPI or damnacanthal resulted in marked reduction of p44 and p42 MAPK activity in a dose dependent manner, suggesting that CD40L induced activation of p44 and p42 is dependent on activity of Lck and Lyn kinases." provenance.
- _5 value "LCK activates Erk1 and Erk2, the activation of Erk2 was greater than Erk1." provenance.
- _5 value "Modified assertion" provenance.
- _4 value "PP1, a general pharmacologic inhibitor of Src kinases (Fyn and Lyn), completely abrogated p44/42 mapk activation by fibrillar Amyloid beta. The accumulation of phospho p44/42 was greatly reduced in both fibrillar Amyloid beta treated Fyn-/- and Lyn-/- macrophages as compared with wild type cells." provenance.
- _4 value "We have previously reported that in thrombin-stimulated human platelets, cytosolic phospholipase A(2) (cPLA2) is phosphorylated on Ser-505 by p38 protein kinase and on Ser-727 by an unknown kinase." provenance.
- _4 value "MEK1 and MEK2 inhibitor, UO126, partially abrogated MCP-1 mRNA induction by Tat, indicating that MEK1 and MEK2 induce the production of MCP-1 modestly." provenance.
- _5 value "Consistent with the physical interaction between ER81 and the coactivators CBP and p300, ER81 transcriptional activity was potentiated by CBP/p300 overexpression." provenance.
- _7 value "HDAC-mediated repression of myogenesis also can be overcome by CaM kinase and insulin-like growth factor (IGF) signaling." provenance.
- _5 value "Corresponding to the accumulation of beta-catenin, expression of the MDR1 gene product was steadily up-regulated in adenomas and adenocarcinomas of 10 patients with familial adenomatous polyposis." provenance.
- _4 value "Tyrosine phosphorylation of STAM2 is induced by growth factors such as epidermal growth factor and platelet-derived growth factor as well as by cytokines like IL-3." provenance.
- _4 value "Tyrosine phosphorylation of STAM2 is induced by growth factors such as epidermal growth factor and platelet-derived growth factor as well as by cytokines like IL-3." provenance.
- _6 value "Using cotransfection experiments, we find that SPBP enhances the transcriptional activity of various transcription factors such as c-Jun, Ets1, Sp1, and Pax6. Hence, SPBP seems to act as a transcriptional coactivator." provenance.
- _5 value "Modified assertion" provenance.
- _3 value "DBP has been directly implicated in the activation of macrophages. Activated macrophages develop the capacity for specialized tasks such as chemotaxis, phagocytosis and the lysis of intracellular parasites, as well as destruction of tumor cells. Lysophosphatidylcholine (lyso-Pc) or dodecylglycerol (DDG) injected into the mouse peritoneum normally activate macrophages to generate reactive oxygen intermediates (ROIs) and to ingest cellular debris during the inflammatory response." provenance.
- _5 value "Studies of the DBP gene proximal promoter have identified three functional hepatocyte nuclear factor 1 (HNF1)-binding sites within 2 kb of the transcriptional start site. The activities of the two more distal HNF1-binding sites were found to be dependent upon the relative levels of the two isoforms of this transcription factor, HNF1a and HNF1b. This was because HNF1b acted as a trans-dominant inhibitor of HNF1amediated enhancer activity14. The relative levels of these HNF1 isoforms in liver and kidney may contribute to the differences in the observed levels of DBP mRNA in these two tissues (Fig. 2). In contrast to their opposing effects on the DBP promoter, HNF1a and HNF1b both display only strong enhancing activities on the AFP and ALB promoters." provenance.
- _4 value "These studies also revealed a rapid increase in GFAT activity by treatment with agents that elevated levels of cyclic adenosine 3',5' monophosphate (cAMP)" provenance.
- _2 value "In this paper, we show that ROS may activate aldose reductase, induce diacylglycerol, activate PKC, induce advanced glycation end product formation, and activate the pleiotropic transcription factor nuclear factor-kappa B (NF-kappaB)" provenance.