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- _4 value "To examine whether HRG signaling results in increased nuclear translocation of beta -catenin, we co-stained control and HRG-activated (30 min) cells with E-cadherin- and beta -catenin-specific antibodies and analyzed by confocal microscopy (Fig. 1D). We found that in addition to an increase within the cytoplasm, there was a significant enhancement of beta -catenin immunostaining inside the nucleus of HRG-treated cells (Fig. 1, lower panel). This was accompanied by cell-shape changes with appearance of some beta -catenin and E-cadherin immunoreactivity at the cellular free edges (Fig. 1D, arrows). In contrast, in control untreated cells, beta -catenin was almost exclusively restricted at the level of intercellular junctions (Fig. 1D, upper panel)." provenance.