Matches in Nanopublications for { ?s ?p ?o <http://purl.org/np/RAPv8Hp5egcpaAp_9Q3KM2WCkRUdUNIKk00vU0gcSyZRw#assertion>. }
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- step type Step assertion.
- _1 label "Cells" assertion.
- _2 label "Cells" assertion.
- step description "Cells can be treated with different antigens for different period of times and the supernatants can be analysed for cytokine levels. The cells can be analysed for phenotypic change, apoptosis or proliferation. Note: PBMCs are primary cells and cannot be cultured for more than one passage under normal conditions. Lymphocytes of PBMCs can be made to proliferate in vitro by mitogens e.g., Phytohaemagglutin or Concanavin-A etc over a period to 72-96 h. Monocytes generally are end cells and do not proliferate. In absence of mitogens the proliferation of PBMCs will be negligible." assertion.
- step bioSampleUsed _2 assertion.
- step hasExperimentalOutput _1 assertion.