Matches in Nanopublications for { ?s <http://www.w3.org/ns/prov#value> ?o ?g. }
- _5 value "Fig. 4 shows the results of the Northern blot analysis, which indicate that endogenous HNF-3? mRNA (indicated with an arrow) was decreased 50% in the CCl4-injured liver compared with the normal rat. However, the transduced HNF-3gamma mRNA (indicated with an arrowhead) was increased dose-dependently by the AxCAHNF3gamma infection in spite of CCl4 treatment. HNF-3alpha and HNF-3beta were up-regulated in the CCl4-treated livers of mock- and AxCALacZ-infected rats. Conversely, These mRNAs in the AxCAHNF3gamma-infected liver were not observed to be induced by CCl4 treatment." provenance.
- _3 value "AGRP is a negative regulator of leptin action" provenance.
- _3 value "injection of leptin reversed the increased AGRP mRNA levels in ob/ob mice but not in db/db mice." provenance.
- _6 value "We conclude that glucose increases AII production by mesangial cells, which results in stimulation of TGF-beta1 secretion, decreased matrix degradation, and increased matrix accumulation. These effects of AII are mediated by the AT1 receptor. Collagenase activity in cell medium was determined using three methods: 1) zymography; 2) quantitative assay using fluoresceinated gelatin as substrate; and 3) a new enzyme-linked immunosorbent assay (ELISA) that specifically measures 72-kDa collagenase (MMP-2), the principal collagenase synthesized by mesangial cells." provenance.
- _3 value "Oncogenic Ras induces the transcription activity of NF-kappaB that confers cell survival. Oncogenic Ras also down-modulates the expression of Par-4, a transcriptional repressor protein, that is essential but not sufficient on its own to induce apoptosis. Here we show that reintroduction of Par-4 by transient transfection leads to apoptosis in cells expressing oncogenic Ras but not in those that lack oncogenic Ras expression. Par-4 abrogates oncogenic Ras-inducible NF-kappaB transcription activity" provenance.
- _5 value "Oncogenic Ras induces the transcription activity of NF-kappaB that confers cell survival. Oncogenic Ras also down-modulates the expression of Par-4, a transcriptional repressor protein, that is essential but not sufficient on its own to induce apoptosis. Here we show that reintroduction of Par-4 by transient transfection leads to apoptosis in cells expressing oncogenic Ras but not in those that lack oncogenic Ras expression. Par-4 abrogates oncogenic Ras-inducible NF-kappaB transcription activity" provenance.
- _5 value "Oncogenic Ras induces the transcription activity of NF-kappaB that confers cell survival. Oncogenic Ras also down-modulates the expression of Par-4, a transcriptional repressor protein, that is essential but not sufficient on its own to induce apoptosis. Here we show that reintroduction of Par-4 by transient transfection leads to apoptosis in cells expressing oncogenic Ras but not in those that lack oncogenic Ras expression. Par-4 abrogates oncogenic Ras-inducible NF-kappaB transcription activity" provenance.
- _8 value "Oncogenic Ras induces the transcription activity of NF-kappaB that confers cell survival. Oncogenic Ras also down-modulates the expression of Par-4, a transcriptional repressor protein, that is essential but not sufficient on its own to induce apoptosis. Here we show that reintroduction of Par-4 by transient transfection leads to apoptosis in cells expressing oncogenic Ras but not in those that lack oncogenic Ras expression. Par-4 abrogates oncogenic Ras-inducible NF-kappaB transcription activity" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "H2320 was transfected (together with pRlSV40) into MCT cells, together with empty expression vector (vector), expression vectors for WT c-Ets2 a mutant c-Ets2 that cannot be phosphorylated by ERK1/2" provenance.
- _3 value "By contrast, G-protein inhibition by pertussis toxin, or transfection with a dominant negative mutant of Ha-Ras strongly increased fibronectin-induced MMP-2 and MMP-9. Inhibition of PI3 kinase, MAPkinase (MEK1), or p38 MAPkinase by wortmannin, PD 98059, or SB 202190, respectively, strongly promoted fibronectin-induced MMP2 and MMP-9" provenance.
- _5 value "By contrast, G-protein inhibition by pertussis toxin, or transfection with a dominant negative mutant of Ha-Ras strongly increased fibronectin-induced MMP-2 and MMP-9. Inhibition of PI3 kinase, MAPkinase (MEK1), or p38 MAPkinase by wortmannin, PD 98059, or SB 202190, respectively, strongly promoted fibronectin-induced MMP2 and MMP-9" provenance.
- _5 value "By contrast, G-protein inhibition by pertussis toxin, or transfection with a dominant negative mutant of Ha-Ras strongly increased fibronectin-induced MMP-2 and MMP-9. Inhibition of PI3 kinase, MAPkinase (MEK1), or p38 MAPkinase by wortmannin, PD 98059, or SB 202190, respectively, strongly promoted fibronectin-induced MMP2 and MMP-9" provenance.
- _5 value "Transient transfection study revealed that p120 enhanced the transcriptional activation of PPARgamma/RXR induced by PPARgamma- or RXR-specific ligands. The yeast two-hybrid analysis showed no interaction of p120 with PPARgamma under any conditions, and electophoretic mobility shift assay showed apparent DNA-PPARgamma/RXR/p120 complex formation only in the presence of 9-cis-RA." provenance.
- _5 value "Transient transfection study revealed that p120 enhanced the transcriptional activation of PPARgamma/RXR induced by PPARgamma- or RXR-specific ligands. The yeast two-hybrid analysis showed no interaction of p120 with PPARgamma under any conditions, and electophoretic mobility shift assay showed apparent DNA-PPARgamma/RXR/p120 complex formation only in the presence of 9-cis-RA." provenance.
- _6 value "Modified assertion" provenance.
- _5 value "Modified assertion" provenance.
- _4 value "We report that trichostatin A (TSA), an inhibitor of histone deacetylases (HDACs), abrogates the ability of p53 to repress the transcription of two genes that it negatively regulates, Map4 and stathmin." provenance.
- _6 value "Here, we report that Ser-727 phosphorylation of Stat3 can also be induced by JNK and activated either by stress or by its upstream kinase and that various stress treatments induce serine phosphorylation of Stat3 in the absence of tyrosine phosphorylation" provenance.
- _5 value "TNF-induced ASK1 activation" provenance.
- _4 value "50 ng/mL TNF-alpha when synergized with 100 U/mL IFN-gamma induced the secretion of IP-10 in endothelial cells. A 3.7 fold increase was observed as compared with IFN-gamma alone. IFN-gamma alone induced the mRNA and protein level." provenance.
- _3 value "A strong induction of Cap43 by okadaic acid" provenance.
- _2 value "These data indicate that Fas-mediated cell death in neuroglioma cells occur, in part, through the production of reactive oxygen species (ROS). These observations also suggest that Fas-induced cell death in these cells occur through apoptosis and necrosis. Thus overexpression of Cu/ZnSOD caused the suppression of both types of Fas-induced cell death" provenance.
- _2 value "Thus, increased GlcN availability induced (a) profound and early inhibition of proximal insulin signaling at the level of PI3K and (b) delayed effects on insulin-mediated glucose uptake, yet (c) complete sparing of insulin-mediated glycogen synthase activation." provenance.
- _4 value "In 3T3-F442A fibroblasts stably expressing the c-fos promoter mutated at the C/EBP binding site upstream of luciferase, c-fos promoter activity is stimulated by GH 6-7-fold; wild type c-fos promoter shows only a 2-fold induction by GH." provenance.
- _3 value "Insulin inhibited the stimulatory effect of a high level of glucose (25 mM) and phorbol 12-myristate 13-acetate, an activator of protein kinase C) on the secretion of ANG and the expression of the ANG messenger RNA in IRPTC." provenance.
- _3 value "Treating asynchronously growing Balb/c 3T3 cells with PP2A-selective concentrations of either okadaic acid or calyculin A caused a time- and dose-dependent decrease in pRB phosphorylation." provenance.
- _6 value "Because Raf binds tightly to the GTP-bound form of Ras but not to the GDP-bound form, such differential affinity can be used to determine the GTP-bound status of Ras. " provenance.
- _7 value "We demonstrate that co-expression of DIP1/2 and the N-terminal domain of DOC-2/DAB2 (i.e. Delta B) has an additive effect on suppressing either TPA-induced SRE or TRE reporter gene activity (Fig. 6B and Table I). Therefore, we believe that the in vivo interaction of DIP1/2 with the N-terminal domain of DOC-2/DAB2 acts as a feedback mechanism to modulate PKC-induced gene activation." provenance.
- _8 value "# Ariadne: We show that p53 wild type and tumor-derived p53 mutations repress C/EBP-mediated transactivation of the albumin promoter. [Expression]" provenance.
- _4 value "Angiotensin II induces the tyrosine and serine phosphorylation of Stat1 and Stat3. Under High Glucose condition both the extent and the duration of phosphorylation of Stat1 and Stat3 was increased." provenance.
- _3 value "In summary, lovastatin and simvastatin predominantly decrease the expression of the glycoprotein thrombospondin-1, which is functionally associated with smooth muscle cell migration and proliferation." provenance.
- _4 value "We also demonstrate that the A --> G mutation increases CDP/Cut binding affinity, and that overexpression of CDP/Cut reduces transcription from TbetaR-II promoter reporter constructs." provenance.
- _3 value "We found increased Erk-1/2 and p38 MAP kinase activities to be important components of ROS-mediated signaling. The pro-oxidant state also contributed to constitutive elevation of AP-1, NFkappaB and cAMP response element transactivation in the malignant phenotype. " provenance.
- _5 value "# Ariadne: Activation of RXR, the partner of PPARgamma, in the presence of rosiglitazone, increased further p85alpha PI-3K and UCP2 mRNA levels and produced a significant augmentation of Glut 4 expression. [Regulation]" provenance.
- _6 value "# Ariadne: In this study we examine the mechanism by which SOCS proteins inhibit GH-induced transcription of the STAT5-responsive Spi 2.1 promoter." provenance.
- _5 value "Recently, Newton and Hogg showed that S100A9 stimulates neutrophil adhesion, and S100A8 reversed the stimulatory effect probably by the formation of the heterodimer." provenance.
- _6 value "Upon elevation of the intracellular calcium level they are translocated from the cytosol to cytoskeleton and to plasma membrane (13)." provenance.
- _8 value "Upon the activation of protein kinase C S100A8/A9 heterodimers are released from human monocytes by a novel secretion pathway that is energy-consuming and depends on an intact microtubule network" provenance.
- _8 value "Upon the activation of protein kinase C S100A8/A9 heterodimers are released from human monocytes by a novel secretion pathway that is energy-consuming and depends on an intact microtubule network" provenance.
- _5 value "TNF-sensitive cells, when exposed to 4-10 microg/ml concentrations of TIP-B1 prior to the addition of TNF, are completely protected from TNF-induced lysis. Furthermore, TIP-B1 protects cells from apoptotic lysis induced by TNF." provenance.
- _5 value "AK1beta,was specifically induced upon activation of wt p53 in Val5 cells" provenance.
- _8 value "Kinase-defective point and deletion variants of RIP2 also significantly blocked the activation of ERK2 by TNFalpha but not epidermal growth factor." provenance.
- _5 value "RIP2 directly phosphorylates and activates ERK2 in vivo and in vitro." provenance.
- _4 value "Ethanol inhibited both PDGF-AA- and PDGF-BB-mediated phosphorylation of PDGFalphar" provenance.
- _5 value "The ubiquitination and subsequent degradation of p27 depend on its phosphorylation by cyclin-CDK complexes." provenance.
- _6 value "The haematopoietic protein tyrosine phosphatase (HePTP) is a negative regulator of the MAP kinases Erk1, Erk2 and p38." provenance.
- _7 value "This modification reduces binding of MAP kinases to the KIM, an effect that is prevented by mutation of serine 23 to alanine." provenance.
- _7 value "This modification reduces binding of MAP kinases to the KIM, an effect that is prevented by mutation of serine 23 to alanine." provenance.
- _7 value "This modification reduces binding of MAP kinases to the KIM, an effect that is prevented by mutation of serine 23 to alanine." provenance.
- _5 value "Epidermal growth factor (EGF), insulin-like growth factor 1 (IGF1) and phorbol myristate acetate (PMA) induce the inhibition of glycogen synthase kinase 3 (GSK3) by stimulating the phosphorylation of an N-terminal serine" provenance.
- _4 value "Epidermal growth factor (EGF), insulin-like growth factor 1 (IGF1) and phorbol myristate acetate (PMA) induce the inhibition of glycogen synthase kinase 3 (GSK3) by stimulating the phosphorylation of an N-terminal serine" provenance.
- _6 value "protein kinase B (PKB) plays a key role in mediating EGF-induced inhibition of GSK3alpha and that the classical MAP kinase (MAPK) cascade has two functions in this process. Firstly, it makes a transient contribution to EGF-induced inhibition of GSK3alpha" provenance.
- _4 value "Modified assertion" provenance.
- _5 value "Whole cell extract of wild-type K562 cells, a transformant cell line of K562 expressing HSF2 and HeLa cells expressing HSF2 transfected with the reporter construct of Cctb gene was found to bind the HSE sequence of Cctb gene, thus activating the Cct gene transcription." provenance.
- _5 value "Whole cell extract of wild-type K562 cells, a transformant cell line of K562 expressing HSF2 and HeLa cells expressing HSF2 transfected with the reporter construct of Cctd gene was found to bind the HSE sequence of Cctd gene, thus activating the Cct gene transcription." provenance.
- _6 value ". IL-18 independently promoted GM-CSF and nitric oxide production , and it induced significant TNF-alpha synthesis by CD14(+) macrophages in synovial cultures; the latter effect was potentiated by IL-12 or IL-15" provenance.
- _4 value ". IL-18 independently promoted GM-CSF and nitric oxide production , and it induced significant TNF-alpha synthesis by CD14(+) macrophages in synovial cultures; the latter effect was potentiated by IL-12 or IL-15" provenance.
- _8 value ". IL-18 independently promoted GM-CSF and nitric oxide production , and it induced significant TNF-alpha synthesis by CD14(+) macrophages in synovial cultures; the latter effect was potentiated by IL-12 or IL-15" provenance.
- _4 value "IL-18 and IL-12 alone induced low levels of IFN- gamma synthesis by cultures of either SF mononuclear cells or synovial membrane?derived single-cell suspensions (Figure 4)" provenance.
- _6 value "IL-18 and IL-12 alone induced low levels of IFN- gamma synthesis by cultures of either SF mononuclear cells or synovial membrane?derived single-cell suspensions (Figure 4)" provenance.
- _5 value "The silencing activity of TIF1alpha appears to result chiefly from histone deacetylation, whereas that of TIF1beta may be mediated via both HP1 binding and histone deacetylation." provenance.
- _4 value "IFN-beta1A enhanced the production of IL4 in MBP-reactive T-cell clones obtained from patients with Multiple sclerosis. IL-4 increased from 42 to 129 pg/ml. IFN-beta1a also enhanced the production of IL4 in Myelin basic protein (MBP)-primed PBMC culture." provenance.
- _6 value "Modified assertion" provenance.
- _3 value "In contrast, 24-h cAMP inhibited PAI-1 gene expression and protein production under basal conditions and in the presence of dexamethasone." provenance.
- _3 value "Insulin acutely up-regulates p85alpha phosphatidylinositol 3-kinase (p85alphaPI 3-K) mRNA levels in human skeletal muscle" provenance.
- _5 value "DOKL and p62(dok) bind through their PTB domains to the Abelson tyrosine kinase in a kinase-dependent manner in both yeast and mammalian cells. DOKL is phosphorylated by the Abl tyrosine kinase in vivo. In contrast to p62(dok), DOKL lacks YxxP motifs in the C terminus and does not bind to Ras GTPase-activating protein (RasGAP) upon phosphorylation. Overexpression of DOKL, but not p62(dok), suppresses v-Abl-induced mitogen-activated protein (MAP) kinase activation but has no effect on constitutively activated Ras- and epidermal growth factor-induced MAP kinase activation. T" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _4 value "A Binding Site for the Transcription Factor Grainyhead/Nuclear Transcription Factor-1 Contributes to Regulation of the Drosophila Proliferating Cell Nuclear Antigen Gene Promoter * Yuko Hayashi , Masahiro Yamagishi , Yoshio Nishimoto , Osamu Taguchi , Akio Matsukage , and Masamitsu Yamaguchi || From the Laboratory of Cell Biology and the Laboratory of Experimental Pathology, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, 464-8681, Japan ABSTRACT TOP ABSTRACT INTRODUCTION EXPERIMENTAL PROCEDURES" provenance.
- _4 value "Fas (CD95 or APO-1), a transmembrane cell surface receptor of the tumor necrosis factor receptor family, is up-regulated in activated T lymphocytes. Our present study identified an upstream enhancer element (between nucleotide positions -862 and -682) containing a GA-binding protein (GABP) site and a low affinity activating protein-1 (AP-1)-binding site. T cell activation increased the DNA binding of GABP and AP-1 to this enhancer site." provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "identified four sites (Ser-124, Thr-308, Thr-450, and Ser-473) on Akt1 that are phosphorylated in vivo. Thr-308 and Ser-473 are inducibly phosphorylated after treatment of cells with extracellular stimuli, whereas Ser-124 and Thr-450 appear to be basally phosphorylated The third mechanism by which 3'-phosphorylated phosphoinositides regulate Akt activity is by controlling the accessibility of Akt as a substrate for PDKs. In in vitro reconstitution assays, the binding of PI3,4,5P to the Akt PH domain is required for PDK-1 to phosphorylate Akt In addition, PDK-1 complexed with either a fragment of PRK2, PRK2, or a PRK2-related peptide may be regulated by increased phospholipid concentrations" provenance.
- _6 value "identified four sites (Ser-124, Thr-308, Thr-450, and Ser-473) on Akt1 that are phosphorylated in vivo. Thr-308 and Ser-473 are inducibly phosphorylated after treatment of cells with extracellular stimuli, whereas Ser-124 and Thr-450 appear to be basally phosphorylated The third mechanism by which 3'-phosphorylated phosphoinositides regulate Akt activity is by controlling the accessibility of Akt as a substrate for PDKs. In in vitro reconstitution assays, the binding of PI3,4,5P to the Akt PH domain is required for PDK-1 to phosphorylate Akt In addition, PDK-1 complexed with either a fragment of PRK2, PRK2, or a PRK2-related peptide may be regulated by increased phospholipid concentrations" provenance.
- _6 value "identified four sites (Ser-124, Thr-308, Thr-450, and Ser-473) on Akt1 that are phosphorylated in vivo. Thr-308 and Ser-473 are inducibly phosphorylated after treatment of cells with extracellular stimuli, whereas Ser-124 and Thr-450 appear to be basally phosphorylated The third mechanism by which 3'-phosphorylated phosphoinositides regulate Akt activity is by controlling the accessibility of Akt as a substrate for PDKs. In in vitro reconstitution assays, the binding of PI3,4,5P to the Akt PH domain is required for PDK-1 to phosphorylate Akt In addition, PDK-1 complexed with either a fragment of PRK2, PRK2, or a PRK2-related peptide may be regulated by increased phospholipid concentrations" provenance.
- _5 value "Dlk can induce apoptosis. In search for interaction partners that might serve as regulators or targets of this kinase we identified apoptosis antagonizing transcription factor (AATF) Interestingly, AATF interfered with Dlk-induced apoptosis." provenance.
- _3 value "On treatment of LNCaP cells with R1881 (a synthetic androgen) it was observed that IGFBP-3 was markedly suppressed both by high and low concentrations of R1881. Levels of IGFBP-3 tended to be lower in biopsies from cancer patients as compared to patients with benign prostatic hyperplasia. It was further found that the marked increase in IGFBP-3 induced by ATRA was largely neutralized by androgen." provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _3 value "Cyclin D1 and D3 mRNA levels decreased during complete differentiation induced by all-trans-retinoic acid and dibutyryl cAMP" provenance.
- _5 value "We here demonstrated that there was a dramatic rise of Id2 and Id3 mRNA levels when 3T3-L1 adipocytes or isolated rat fat cells were exposed to lipolytic and anti-lipogenic agents, forskolin and isoproterenol." provenance.
- _4 value "jubilant" provenance.
- _3 value "1alpha,25-dihydroxyvitamin D(3) (D(3)) promotes the maturation of myeloid cells and surface expressions of CD14 and CD11b, markers of cell differentiation in response to D(3)." provenance.
- _5 value "Modified assertion" provenance.
- _3 value "mRNA encoding ADD-1/SREBP-1c was suppressed by fasting and restored by feeding in adipose tissue of the mouse, and that this closely paralleled the expression of two adipocyte genes that are likewise regulated by nutrition, FAS, and leptin." provenance.
- _3 value "Culturing the cells in lipoprotein-deficient serum for 2 days decreased their total cholesterol concentration by 25% (from 68 to 51ug cholesterol/mg protein) but did not significantly alter the ORP-1 mRNA levels. However, when the partially delipidated cells were loaded with LDL for 24 h, the ORP-1 transcript was up-regulated by 2-fold (Fig. 5). Simultaneously, the cellular cholesterol level returned back to normal (not shown). Short-term treatment of the sterol-depleted cells with 25-hydroxycholesterol (5 ug/ml of 25-hydroxycholesterol for 4 h), a condition known to stimulate the translocation of OSBP to the Golgi apparatus (10, 13), had a minor (1.5-fold) stimulatory effect on the expression of the ORP-1 mRNA (Fig. 5)." provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _6 value "Modified assertion" provenance.
- _3 value "nerve growth factor overexpressing keratinocytes were partially resistant to apoptosis induced by increasing doses of ultraviolet B" provenance.
- _3 value "ultraviolet B radiation downregulated nerve growth factor mRNA and protein" provenance.
- _5 value "Modified assertion" provenance.
- _5 value "Modified assertion" provenance.
- _5 value "Recombinant tagged PHI-1 was phosphorylated by protein kinase C at two sites, one a Ser and one a Thr; phosphorylation enhanced inhibitory potency 50-fold." provenance.
- _4 value "Inhibition of ecNOS activity in MCF-7 cells increased tumor cell apoptosis" provenance.
- _4 value "AP-1-dependent genes, such as collagenase (MMP-13) and stromelysin (MMP-3)" provenance.
- _4 value "It regulates proliferation, differentiation, and maturation of erythroid cells." provenance.
- _6 value "All five cell lines did not express FADD by immunoblot analysis and were completely resistant to Fas-induced death. Complementation of the FADD mutant cell lines with wild-type FADD restored Fas-mediated apoptosis. Fas activation of caspase-2, caspase-3, caspase-7, and caspase-8 and the proteolytic cleavage of substrates such as BID, protein kinase Cdelta, and poly(ADP-ribose) polymerase were completely defective in the FADD mutant cell lines. In addition, Fas activation of the stress kinases p38 and c-Jun NH2 kinase and the generation of ceramide in response to Fas ligation were blocked in the FADD mutant cell lines" provenance.
- _4 value "We report that TNF induces DNA replication in growth-arrested LE6 cells and that its effect involves the activation of NFkappaB and STAT3 and an increase in c-myc and IL-6 mRNAs. We conclude that NFkappaB is an essential component of the TNF proliferative pathway and that TNF-induced changes in IL-6 mRNA, STAT3, and c-myc mRNA are dependent on NFkappaB activation." provenance.
- _5 value "MKP-1 was a target in vivo and in vitro for p42(MAPK) or p44(MAPK), which phosphorylates MKP-1 on two carboxyl-terminal serine residues, Serine 359 and Serine 364." provenance.
- _5 value "PhosphoElm data from PMID 15212693" provenance.
- _5 value "Myogenin expression, and not MyoD, was found to up-regulate MEF2C expression. MEF2A, -B, and -D expression levels were not up-regulated by overexpression of either MyoD or myogenin. To examine whether MEF2C can differentially regulate MyoD or myogenin expression, P19 cell lines overexpressing MEF2C were analyzed. MEF2C induced myogenesis in P19 cells and up-regulated the expression of myogenin with 25-fold greater efficiency than that of MyoD. Therefore, myogenin and MEF2C participate in a regulatory loop in differentiating stem cells." provenance.