Matches in Nanopublications for { <http://purl.org/np/RAysi2SPEKm8TaYscHjRtWBr9tT0a2IA9d0jhRLRDP8bc#step> ?p ?o ?g. }
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- step type Step assertion.
- step description "Ground samples (leaf, shoot, root, and recalcitrant samples, approximately 0.5-1 g) using 1 ml of the extraction buffer with or without liquid nitrogen in mortar and pestle that are sterilized.CRITICAL STEP The procedure are carried out at room temperature except the centrifugation steps (at 4℃) as well as the time of precipitating of the nucleic acid using the isopropanol (on ice).CRITICAL STEP Severely disrupt the tissues to create the glaze mode of samples." assertion.
- step isStepOfPlan plan assertion.
- step precedes step assertion.
- step reagentUsed _5 assertion.
- step reagentUsed CHEBI_17997 assertion.
- step labEquipmentUsed _3 assertion.
- step labEquipmentUsed _4 assertion.
- step bioSampleUsed EFO_0000992 assertion.
- step bioSampleUsed PO_0025034 assertion.
- step bioSampleUsed PO_0009005 assertion.
- step bioSampleUsed _2 assertion.
- step hasExperimentalInput EFO_0000992 assertion.
- step hasExperimentalInput PO_0025034 assertion.
- step hasExperimentalInput PO_0009005 assertion.
- step hasExperimentalInput _1 assertion.