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- _2 value "Secondary insulin resistance in liver and adipose tissue Steady-state circulating free fatty acid (FFA) levels represent a balance between FFA production and removal. The acute effects of insulin in lowering FFA levels are due, in large part, to insulin's antilipolytic effects in adipose tissue. In control animals, insulin infusion suppressed FFA levels by approx60% in both 6- and 14-month-old mice (Fig. 4a). This effect was significantly blunted in 6- and 14-month-old MKOfl/fl (P = 0.0089 and P = 0.01, respectively) mice, as well as 14-month-old MKOfl/- mice (P = 0.009), as fatty acid levels in those mice were depressed by only approx30%." provenance.