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- _7 value "The β-casein promoter, known to be induced by Stat5 when activated by prolactin via prolactin receptors and JAK2, was next used to test the function of the phosphorylated Stat5. T47D-YB cells were pretreated with either R5020 or ethanol vehicle for 48 h. Cells were then washed, transfected with a β-casein promoter-luciferase reporter, treated with either R5020 or prolactin, and harvested for luciferase assay 24 h after hormone treatment. We found that in T47D-YB breast cancer cells, prolactin alone has minimal to no significant effect on the β-casein luciferase promoter. However, after R5020 pretreatment, a 6–7-fold induction of the β-casein luciferase promoter was observed (Fig.7B). Thus R5020 sensitized these breast cancer cells to the transcriptional effects of prolactin mediated through Stat5." provenance.