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- _5 value "Treatment with CSE for 24 hours increased GRP78 protein in concentration-dependent fashion (P , 0.005) (Figure 6). The maximum increase was observed at CSE concentrations of 15 to 30% CSE. CSE extract (30%) also rapidly increased phosphorylation of eIF2a, a target of PERK, without affecting total eIF2a (Figure 7A). CSE (30%) also increased nuclear expression of the PERKregulated transcription factors, ATF4 and Nrf2 (Figure 7B)." provenance.