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- _5 value "Primary cultured human saphenous vein ECs had negligible levels of Cat S mRNA. Exposure to IFNG, IL-1A, or TNF-A caused accumulation of Cat S transcript. However, the angiogenic factors VEGF and bFGF had limited effects on Cat S transcription (Figure 1A). For detection of Cat S protein in ECs, [125I]JPM ethyl ester, the cell-permeable active site probe for cysteine proteases, was used (Figures 1B and 1C). This irreversible probe labels Cat S immediately on activation and does not affect Cat S immunoreactivity.8 IFNG increased EC secretion of active Cat S 20-fold (mean of three independent experiments by densitometry measurement; Figure 1B, left). Cell-associated Cat S increased in parallel (Figure 1C). VEGF and bFGF also induced Cat S secretion up to 20- and 3-fold, respectively (mean of three independent experiments; Figure 1B, right)." provenance.