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- _5 value "These findings suggest that the capability of CTCF to suppress PAX6 transcription is dependent on its ability to bind to its binding motifs in the 80-bp region upstream from the PAX6 P0 promoter.To determine the dependence of CTCF DNA binding on these five CCCTC binding motifs in the 80-bp region upstream from the PAX6 P0 promoter, each of these motifs mutated from CCCTC to GGATC and was tested in EMSA binding and competing experiments. multiple mutations of CTCF-binding motifs in different combinations in the 80-bp sequence markedly decreased CTCF binding activity in nuclear extracts and CTCF binding activity with mutant probes was competitively inhibited by the respective multimutated cold probes (Figs. 5G 5H) . These results suggest that five potential CTCF-binding motifs in the 80-bp region are all involved in CTCF DNA binding." provenance.