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- _3 value "Table 4 Relative expression (RE) of 29 selected cellular genes in four study models by real-time RT-PCR - see comparisons below: Model 2 (M2) values were normalized against the untreated control macrophages of model 1 (M1) at 24 h. Model 3 (M3) values were normalized against M1 at 72 h. Models 4 and 5 (M4 and M5) were normalized against M1 at 96 h. M1@24, M1@72 and M1@96 denote control untreated macrophages whose RNAs were harvested and analyzed at 24, 72 and 96 h, respectively. M2@24 denotes LDL-treated macrophages with RNA analyzed at 24 h. M3@72 denotes macrophages infected with C. pneumoniae and RNA analyzed at 72 h. M4@96 denotes macrophages treated with LDL for 24 h, followed by C. pneumoniae infection for another 72 h before analyzing RNA at 96 h. M5@96 denotes macrophages infected with C. pneumoniae first for 72 h, followed by LDL treatment for another 24 h before analyzing RNA at 96 h. {for M4 and M5, results were combined and only the overlapping gene changes were modeled} (from methods) The two-tailed t-test was then conducted to ascertain the statistical significance of the differences between the normalized relative expression values for each gene under the conditions stipulated for study models 2, 3, 4 and 5" provenance.