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- _5 value "To confirm that the JNK-dependent TNF-induced cell death was not apoptosis, we examined the morphology of the cells by electron microscopy (Fig. 4). TNF-treated wild-type (N-IkBa) cells displayed altered morphology, including the presence of distended cytoplasmic organelles and rupture of nuclear and plasma membranes, in the absence of obvious apoptotic bodies. These data indicate that the JNK-dependent TNF-stimulated cell death observed in wild-type (N-IkBa) cells is mediated, at least in part, by a nonapoptotic (necrotic) mechanism." provenance.