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- _6 value "Phosphorylation of both ERKs remained high for 2 h in the T47D/puro cells, whereas it decreased to the basal level in the T47D/5R cells after 2 h.....More striking differences between the two cell lines were observed when cells were stimulated with NDF (Fig. 4B).....Therefore, we conclude that both EGF- and NDF-induced MAPK activation is impaired by the absence of cell surface ErbB-2." provenance.