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- _5 value "1 h of 10-Hz train stimulation significantly increased (P < 0.01) the mean nuclear stain of activated CaMKII compared with unstimulated fibers, and KN-62 (5 μM) blocked the increase in activated CaMKII staining after stimulation (Fig. 3 A). In parallel experiments, in which the fibers were stained with antibody directed against total CaMKII, there was no difference in mean nuclear antibody stain between stimulated and unstimulated fibers (P > 0.05; Fig. 3 A). These results are consistent with activation of nuclear CaMKII by fiber electrical stimulation without any appreciable translocation of CaMKII from the cytoplasm to the nucleus." provenance.