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- _4 value "Viral-induced dimerization of IRF3 (IFR3 dimers are transcriptionally active), as well as phosphorylation of STAT1 , was abrogated in RIG-/- MEFs (Figures 4A and 4B). Furthermore, electrophoretic mobility shift assay (EMSA) revealed that activation of IFN stimulated a regulatory element (ISRE), and NF-kB activity was also abrogated in RIG-I-/- cells in response to viral infection (Figures 4)" provenance.