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- _4 value "A relatively small number of genes was up-regulated in E2f6-/- cells when compared with wild-type control cells (data not shown). Activation of genes in E2f6-/- cells is consistent with the proposed role for E2F6 in transcriptional repression. In this study we focused on two genes that were strongly up-regulated in E2F6-deficient cells and that encode for STAG3 and SMC1{beta}, meiotic specific subunits of the cohesin complex (19–21). Both proteins are required for proper segregation of chromosomes during meiotic division. Expression of a third gene, XM_196054, was also increased in E2f6-/- cells. This cDNA encodes for a novel protein with an unknown function. To validate the array data we analyzed the expression of these genes in different MEF preparations by RT-PCR. STAG3, SMC1{beta}, and XM_196054 were undetectable in wild-type fibroblasts and strongly elevated in E2f6-/- MEFs, confirming the microarray data (Fig. 1A)." provenance.