Matches in Nanopublications for { <http://www.tkuhn.ch/bel2nanopub/RAJNhw19XPmkum7Nd3rDSa54KNcxg1Z7OUZ5HMWLAvBkQ#_5> ?p ?o ?g. }
Showing items 1 to 2 of
2
with 100 items per page.
- _5 wasQuotedFrom 15849359 provenance.
- _5 value "Using mass spectrometry, we identified Ser(24) in the pleckstrin homology (PH) domain of IRS-1 as a specific phosphorylation site for mPLK. IRS-1 mutants S24D or S24E (mimicking phosphorylation at Ser(24)) had impaired ability to associate with insulin receptors resulting in diminished tyrosine phosphorylation of IRS-1 and impaired ability of IRS-1 to bind and activate PI-3 kinase in response to insulin. Wild-type mPLK, but not a kinase-inactive mutant (mPLK-KD), directly phosphorylated full-length IRS-1 in vitro. This in vitro phosphorylation was increased when mPLK was immunoprecipitated from tumor necrosis factor (TNF)-alpha-treated cells." provenance.