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- _4 value "As illustrated in Fig.2, A–C, the expression of E-selectin correlated with an increased adhesion of both colon carcinoma HT-29 cells and HL-60 leukemia cells to a monolayer of HUVEC. After 30 min, the number of HT-29 cells that adhered to HUVEC-expressing E-selectin, following activation with TNFα, was 5-fold higher than when adhering to inactivated HUVEC. Similarly, HT-29 cells quickly adhered to immobilized recombinant human E-selectin/Fc chimera (data not shown). An anti-E-selectin neutralizing antibody, but not a matched isotype antibody, decreased the adhesion of both cancer cell types to the activated endothelium (Fig. 2, A–C)." provenance.