Matches in Nanopublications for { <http://www.tkuhn.ch/bel2nanopub/RAOC96MCVA7v9sAkeL-M5i6z5jLcwTqgPrb7T79SyFsw8#_3> ?p ?o ?g. }
Showing items 1 to 2 of
2
with 100 items per page.
- _3 wasQuotedFrom 17105752 provenance.
- _3 value "Gastrin, a potent stimulator of gastric acid secretion, primarily targets the acid-secreting parietal cells and histamine-secreting enterochromaffin like (ECL) cells in the stomach. Accordingly, gastrin-deficient (GAS-KO) mice have a severe impairment in acid secretion. The aim of this study was to characterize changes in gene expression in GAS-KO mice to identify gastrin-regulated genes and to gain insight into how gastric cell types are regulated by gastrin and acid secretion. Affymetrix microarray analysis of GAS-KO and wild type mice identified numerous differentially expressed transcripts. The results were compared to GAS-KO mice treated with gastrin to identify genes that were gastrin-responsive. Finally, genes that were primarily changed due to gastrin and not hypochlorhydria were identified by comparison to mice that are deficient in both gastrin and cholecystokinin (GAS/CCK-KO), since these mice have restored basal acid secretion. The data were validated by quantitative reverse transcriptase polymerase chain reaction analysis. Interestingly, a number of inflammatory response genes were induced in GAS-KO mice and normalized in GAS/CCK-KO mice, suggesting that they were increased in response to low gastric acid. Moreover, a number of parietal cell transcripts that were down-regulated in GAS-KO mice were similarly restored in GAS/CCK-KO mice, suggesting that parietal cell changes were also primarily associated with hypochlorhydria. In contrast, ECL cell genes that were markedly down-regulated in GAS-KO mice continued to be reduced in GAS/CCK-KO mice, demonstrating that gastrin coordinately regulates a number of ECL cell genes, including several involved in histamine synthesis and secretion. Key words: parietal cell, enterochromaffin-like (ECL) cell, cholecystokinin (CCK), parathyroid hormone-related protein (Pthlh), inflammation." provenance.