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- _5 wasQuotedFrom 12524439 provenance.
- _5 value "PRAS40 was phosphorylated in vitro by purified Akt on the same site that was phosphorylated in insulin-treated cells. (From Results section) -- To determine whether the predicted Akt consensus phosphorylation site (Thr-246) was in fact the site recognized by the anti-pAkt substrate antibody, a mutant form of the HA-tagged PRAS40 (T246A) was expressed in which this site was changed to Ala. The anti-pAkt substrate did not recognize the mutant PRAS40, even when the constitutively active Akt was co-expressed with this protein (Fig. 5). --" provenance.