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- _4 wasQuotedFrom 19657053 provenance.
- _4 value "HMVECs were transfected with Rac siRNA as described in materials and methods in both normoglycemic (A) and hyperglycemic (B) conditions. Both transfected and nontransfected HMVECs were rendered quiescent and then stimulated with 100 nM FTY720 for 3 h. Cell lysates were probed with anti-FAK [Y576] phosphospecific antibody and subsequently reprobed with anti-FAK antibody. Representative blots of 3 independent experiments are shown...Rac protein depletion by small interfering RNA (siRNA) technique completely abolished FTY720-induced FAK [Y576] phosphorylation." provenance.