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- _5 value "To test this hypothesis, whole cell protein extracts isolated fromvehicle- and LPS-treated AEBP1+/+ and AEBP1?/? macrophages were subjected to SDS-PAGE followed by immunoblotting (Fig. 3A). Histograms illustrating relative protein levels of PPAR1 and LXR normalized based on actin expression are shown in Fig. 3B. As shown in Fig. 3A and B, although LPS significantly inhibits PPAR1 and LXR expression in AEBP1+/+ macrophages, AEBP1 ablation almost completely interferes with LPS-induced suppression of PPAR1 and LXR expression (80% versus 20% reduction, respectively, 48 h post LPS treatment)." provenance.