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- _5 wasQuotedFrom 9813040 provenance.
- _5 value "T47Dco cells were treated for 24 h with 10 nm R5020 or vehicle, then with 10 nm EGF or vehicle for 5 min. Nuclear and cytosolic extracts were prepared and equal amounts of protein were probed with antibody recognizing total Stat5 (Fig. 4A). In untreated cells, Stat5 was exclusively cytoplasmic (lane 1); no Stat5 was found in the nucleus (lane 2). As expected, EGF treatment promoted nuclear translocation of a portion of Stat5 (lanes 5 and 6). Surprisingly, R5020 not only increased total Stat5 levels (lanes 3 and 4; 7and 8), but also promoted extensive nuclear translocation of Stat5 (compare lanes 2 and 4) independent of EGF treatment." provenance.