Matches in Nanopublications for { <http://www.tkuhn.ch/bel2nanopub/RAb_Apuh0jczRG4WtM_l5Q1MsjyS503SgG26DldA4GCGw#_4> ?p ?o ?g. }
Showing items 1 to 2 of
2
with 100 items per page.
- _4 wasQuotedFrom 12796479 provenance.
- _4 value "We first determined whether Rac-1 is activated in NIH-3T3 cells allowed to adhere after presuspension. In agreement with previous reports (del Pozo et al., 2000), we observed an early accumulation of GTP-bound (i.e., active) Rac-1 upon cell plating on fibronectin with a kinetic comparable with the generation of oxygen species (Fig. 3 A). A causal link between Rac activation and adhesion-induced ROS was further demonstrated by overexpressing constitutively active Rac-1 mutant (RacQL) or the corresponding dominant-negative form (RacN17) of the GTPase (Fig. 3 B). As shown in Fig. 3 C, Rac-1?overexpressing cells plated on fibronectin produce significantly more hydrogen peroxide than cells in suspension. " provenance.