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- _4 wasQuotedFrom 17260024 provenance.
- _4 value "To confirm the in vivo interaction between the miR-15a/miR-16-1 cluster and Bcl-2 in NB4 cells, we transfected the precursor miR-16- 1 (pre-miR-16-1) oligonucleotide or scrambled oligonucleotides into NB4 cells by using nucleoporation and obtained total RNA at 24 h and proteins from whole cell lysates at 48 h. As shown in Figure 5c, there was a significant reduction of the Bcl-2 transcripts by qRT– PCR after transfection with pre-miR-16-1. Likewise, transfection of the pre-miR-16-1, but not the control, resulted in a strong Bcl-2 protein reduction by Western blots at 48 h (Figure 5e)." provenance.