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- _6 value "Overexpression of a constitutively active HA-tagged Akt mutant (Ad-Akt(Myr)), but not GFP, significantly attenuated IL-18-mediated HCMEC death (Fig. 3D—expression of HA was confirmed by Western blotting, right-hand panel). Furthermore, transfection with the expression vector for the anti-apoptotic gene Bcl-2, but not the empty vector, also attenuated IL-18-mediated cell death (Fig. 3D). Together, these results indicate that PTEN enhanced whereas Akt and bcl-2 antagonized IL-18-mediated cell death (Fig. 3)." provenance.