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- _4 value "In Lmx1b-/- embryos, Drg11 expression was completely abolished from the normal onset of its expression (Fig. 3A,B; see Fig. S1 at http://dev.biologists.org/supplemental). At E11.5, Ebf1, Ebf2, Ebf3 and Rnx appeared to be normally expressed in Lmx1b-/- embryos (see Fig. S1 at http://dev.biologists.org/supplemental). However, by E12.5, Ebf3 and Rnx expression levels were lower in Lmx1b mutants (see Fig. S1) and by E15.5, Ebf3 expression was completely absent in the Lmx1b mutants (Fig. 3C,D). At this stage, in wild-type embryos, Ebf1 was most strongly expressed in laminae III neurons and weakly in laminae III-IV neurons. By contrast, Ebf1 expression was markedly reduced in Lmx1b mutant embryos (Fig. 3E,F). Similarly, Rnx was dramatically downregulated in the Lmx1b mutants (Fig. 3G,H)." provenance.