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- _5 value "Figure 3A provides a gel shift analysis in which nuclear extracts isolated from bFGF-treated pulmonary fibroblast cultures were incubated with radiolabeled serum response element (SRE) of the c-Fos promoter. Several major complexes form, including two slow migrating complexes (complexes A and B) and several faster moving complexes (complex C). The addition of phosphorylated Elk-1 antibody abrogates the binding of complex B, demonstrating that phosphorylated Elk-1 is a component of complex B and, more importantly, showing that bFGF treatment results in the phosphorylation of Elk-1 (Fig. 3B). " provenance.