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- _6 value "We next examined whether mutations of this MEF2 site affects MEF2 activity on the PGC-1α promoter. The mutated 2-kb promoter (called ΔMEF2; see Fig. 3A) is no longer able to mediate MEF2C or MEF2D induction alone, when activated by CnA or coactivated by PGC-1α, suggesting that this site is responsible for the MEF2 action (Fig. 3B). Moreover, electrophoretic mobility-shift assays revealed binding of MEF2C to this MEF-binding site but not the mutated ΔMEF site in the PGC-1α promoter (Fig. 3C). The identity of the MEF2C–DNA complex was confirmed by using an anti-MEF2 antibody that leads to a supershift of the DNA–protein complex." provenance.