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- _7 value "# Ariadne: We have also observed in vivo interactions between Smad2 and MEF2A using co-immunoprecipitation assays. [Binding] Our studies indicate that Smad2 and 4 (Smad2/4) complexes cooperate with MEF2 regulatory proteins in a GAL4-based one-hybrid reporter gene assay. # Ariadne: We have also observed in vivo interactions between Smad2 and MEF2A using co-immunoprecipitation assays. [Binding]" provenance.