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- _7 value "Finally, to confirm that dnRab5 and dn-dynamin expression impacted cell proliferation in a manner similar to Rin1 depletion, A549 cells transiently transfected with expression constructs encoding GFP-dnRab5, GFP-dn-dynamin, or GFP alone were tested for their ability to form colonies (Fig. 8). Cells transfected with GFP-dnRab5 or GFP-dn-dynamin expression constructs showed decreased cell proliferation, supporting the conclusion that proper EGFR internalization and Rab5-mediated endosomal trafficking of EGFR are critical for potentiating proliferative signaling in A549 cells." provenance.