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- _5 value "For this reason we examined the effects of stress on AKT2 activation in two ovarian epithelial cancer cell lines, A2780 cells, which were transiently transfected with HA-AKT2, and OVCAR3 cells, which express high levels of endogenous AKT2 (7). The cells were exposed to UV-C, heat shock (45 °C), 0.4 M NaCl, or 20 ng/ml TNFalpha. IGF1- stimulated cells were used as controls. As assessed by in vitro kinase and Western blot analyses of AKT2 immunoprecipitates, all the stimuli substantially increased AKT2 activity in both A2780 and OVCAR3 cells (Figs. 1, A and B)." provenance.