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- _5 value "In contrast, at 10 days postbirth ANF and alpha -skeletal actin mRNA levels were higher by factors of 5.2 ± 0.6 and 5.8 ± 1.3 in Irx4Delta ex2/Delta ex2 hearts than in wild-type hearts, respectively (Fig. 2A). At 6 weeks, beta MHC mRNA levels were also increased (by a factor of 2.1 ± 0.2 versus the wild-type level). By 24 weeks, Irx4Delta ex2/Delta ex2 hearts contained increased levels of ANF (factor of 6.7 ± 1.3), BNP (factor of 4.3 ± 0.6), alpha -skeletal actin (factor of 5.4 ± 0.8), and beta MHC (factor of 2.7 ± 0.15) mRNAs compared to mRNA levels in wild-type hearts (Fig. 2A). " provenance.