Matches in Nanopublications for { <http://www.tkuhn.ch/bel2nanopub/RAt8XQdKJ8tc3pnjAZ0vFfGIExAJVvNSiygAHD_HsUlMA#_5> ?p ?o ?g. }
Showing items 1 to 2 of
2
with 100 items per page.
- _5 wasQuotedFrom 15257658 provenance.
- _5 value "To understand how cells control E2F6 expression, we analysed the activity of the previously cloned promoter region of the human E2F6 gene. DNase I footprinting, gel electrophoreticmobility shift, transient transfection and site-directed mutagenesis experiments allowed the identification of two functional NRF-1/alpha-PAL (nuclear respiratory factor-1/alpha-palindrome-binding protein)-binding sites within the human E2F6 core promoter region, which are conserved in the mouse and rat E2F6 promoter region. Moreover, ChIP (chromatin immunoprecipitation) analysis demonstrated that overexpressed NRF-1/alpha-PAL is associated in vivo with the E2F6 promoter. Furthermore, overexpression of full-length NRF-1/alpha-PAL enhanced E2F6 promoter activity, whereas expression of its dominant-negative form reduced the promoter activity. Our results indicate that NRF-1/alpha-PAL is implicated in the regulation of basal E2F6 gene expression." provenance.