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- _5 wasQuotedFrom 7852366 provenance.
- _5 value "We cotransfected pMRF4.CAT into 10T1/2 fibroblasts along with activator plasmids encoding one of the four myogenic bHLH factors or MEF2A, MEF2C, or MEF2D. As shown in Fig. 3, myogenin, myf5, and MyoD all mediate a strong trans-activation (about 20-fold) of the MRF4 promoter. In contrast, MRF4 was unable to activate its own promoter in this assay. All of the MEF2 factors tested were able to reproducibly activate the MRF4 promoter from 4-10-fold." provenance.